Software tools for automated transmission electron microscopy

Diagnostic Transmission Electron Microscopy

The high resolution and sensitivity of electron microscopy is still a valuable tool, and for some diseases it is the gold standard in pathological diagnosis. Today the sample turnaround time for processing in the lab can be significantly reduced from days to hours by the microwave technology. In urgent clinical cases, microwaveassisted tissue processing, in combination with digital image acquis...

Conventional transmission electron microscopy

Researchers have used transmission electron microscopy (TEM) to make contributions to cell biology for well over 50 years, and TEM continues to be an important technology in our field. We briefly present for the neophyte the components of a TEM-based study, beginning with sample preparation through imaging of the samples. We point out the limitations of TEM and issues to be considered during ex...

Electron Diffraction Using Transmission Electron Microscopy

Electron diffraction via the transmission electron microscope is a powerful method for characterizing the structure of materials, including perfect crystals and defect structures. The advantages of electron diffraction over other methods, e.g., x-ray or neutron, arise from the extremely short wavelength (≈2 pm), the strong atomic scattering, and the ability to examine tiny volumes of matter (≈1...

Uniform serial sectioning for transmission electron microscopy.

Kristen M. Harris,1,2,3 Elizabeth Perry,2 Jennifer Bourne,1,2 Marcia Feinberg,3 Linnaea Ostroff,3 and Jamie Hurlburt2,3 1Section of Neurobiology and Center for Learning and Memory, University of Texas at Austin, Austin, Texas 78712, 2Department of Neurology, Synapses, and Cognitive Neuroscience Center, Medical College of Georgia, Augusta, Georgia 30912, 3Program in Neuroscience and Biology Depa...

Scanning Transmission Electron Microscopy for Nanostructure Characterization